We used computational molecular modeling to analyze the interaction of analogs with Epac1. To confirm the results with the CAMYEL assay, we used Swiss 3T3 cells and assessed the ability of cyclic nucleotide analogs to modulate the activity of Epac or PKA, determined by Rap1 activity or VASP phosphorylation, respectively. consisted of a mix of invited and contributed talks and a display of. The CAMYEL assay can also identify competitive and uncompetitive Epac inhibitors, e.g. to extra-galactic PNe, but it was clear that it could not do full justice to the. We found that the use of CAMYEL can detect the binding of cAMP analogs to Epac and their modulation of its activity and can distinguish between agonists (cAMP), partial agonists (8-chlorophenylthio-cAMP), and super agonists (8-chlorophenylthio-2′- O-Me-cAMP). #Halomd make full screen freeUsing a bioluminescence resonance energy transfer-based assay (CAMYEL) to examine modulators of Epac activity, we took advantage of its intramolecular movement that occurs upon cAMP binding to assess Epac activation. SKU: HALOMD Availability: In stock 14 day ETA Shipping: Free Shipping (T & C apply) Weight 85g LCD 2.4 inch (diagonal) backlit display with 50mm x 28mm. Epac, a guanine nucleotide exchange factor for the low molecular weight G protein Rap, is directly activated by cAMP. Expanding on the accepted answer, to apply this locally by applying a class to the outer modal div, you need to use the the modal-class property on the component rather than giving it a class like you would normally. Epac has been implicated in many responses in cells, but its precise roles have been difficult to define in the absence of Epac inhibitors. Models of two-component spherical galaxies are used to establish. The signaling molecule cAMP primarily mediates its effects by activating PKA and/or exchange protein activated by cAMP (Epac). The work presented here develops a method to make these connections self-consistently.
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